WT UV-DDB Performs a 3D Search on DNA whereas the XP-E Mutant (K244E DDB2) Mutant Slides
نویسندگان
چکیده
منابع مشابه
Single-molecule analysis reveals human UV-damaged DNA-binding protein (UV-DDB) dimerizes on DNA via multiple kinetic intermediates.
How human DNA repair proteins survey the genome for UV-induced photoproducts remains a poorly understood aspect of the initial damage recognition step in nucleotide excision repair (NER). To understand this process, we performed single-molecule experiments, which revealed that the human UV-damaged DNA-binding protein (UV-DDB) performs a 3D search mechanism and displays a remarkable heterogeneit...
متن کاملXP mutant power!
Diabetic eye damage may start in bone marrow, suggest Busik and colleagues. Damaged bone marrow nerves and disrupted circadian genes hampered the release of progenitor cells that are required to repair diabetes-induced vessel injury in the eye. Up to 45% of diabetic adults in the US develop retinopathy, a potentially blinding condition. Although high glucose levels and oxidative stress may caus...
متن کاملBrg1 Wt Brg1 Mutant
(71) Applicants:Zainab JAGANI, Cambridge, MA (US); EPE" application No. 61/846,178, s s s Jul 15, 2013. Gregory HOFFMAN, Cambridge, MA ed. On Jul. 1, (US); Frank Peter STEGMEIER, Publication Classification Cambridge, MA (US); Craig Stephen MICKANIN, Cambridge, MA (US) (51) Int. Cl. CI2O I/68 (2006.01) (72) Inventors: Zainab JAGANI, Cambridge, MA (US); GOIN33/574 (2006.01) Gregory HOFFMAN, Cambr...
متن کاملThe naturally occurring mutants of DDB are impaired in stimulating nuclear import of the p125 subunit and E2F1-activated transcription.
The human UV-damaged-DNA binding protein DDB has been linked to the repair deficiency disease xeroderma pigmentosum group E (XP-E), because a subset of XP-E patients lack the damaged-DNA binding function of DDB. Moreover, the microinjection of purified DDB complements the repair deficiency in XP-E cells lacking DDB. Two naturally occurring XP-E mutations of DDB, 82TO and 2RO, have been characte...
متن کاملThe DDB1-CUL4ADDB2 ubiquitin ligase is deficient in xeroderma pigmentosum group E and targets histone H2A at UV-damaged DNA sites.
Xeroderma pigmentosum (XP) is a heritable human disorder characterized by defects in nucleotide excision repair (NER) and the development of skin cancer. Cells from XP group E (XP-E) patients have a defect in the UV-damaged DNA-binding protein complex (UV-DDB), involved in the damage recognition step of NER. UV-DDB comprises two subunits, products of the DDB1 and DDB2 genes, respectively. Mutat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2013
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2012.11.462